欢迎来到蚂蚁淘！(生命科学科研用品平行进口B2B商城)

首页
我的蚂蚁淘
快速下单
产品分类
网站导航
- 特色主题 -
  
  秒杀抢购新品试用购物有礼精品推荐蚁豆商城品质团购活动专题 "1"元拍卖
- 蚂蚁淘 +
  
  体验官万众创业品牌合作我在蚂蚁淘
- 行业信息
  
  新闻资讯蚂蚁淘知道技术文章
- 客户服务
  
  帮助中心在线客服购物指南找货助手
关注我们
联系我们

Bimake

主营：分子类，蛋白类，抗体类，生化类试剂

当前位置：首页 > 产品中心 > > Bimake/All-in-One cDNA Synthesis SuperMix/200 rxns/B24403

公司介绍

产品分类

更多…

销售排行

更多…

问答列表

更多…

B24403

产品编号： B24403
美元价： $300.00
市场价： ¥6000.00
品牌： Bimake
产地：美国(厂家直采)
公司： Bimake
产品分类：仪器类>温控控制>PCR仪>
公司分类：

联系 Q Q: 1570468124
电话号码: 4000-520-616
邮箱: info@ebiomall.com

Bimake/All-in-One cDNA Synthesis SuperMix/200 rxns/B24403

商品介绍

Advantages
Customer Reviews
Product Comparison
Price Comparison
Description
File Download

Advantages

Reproducibility: Modified Oligo dT: Random primers ratio facilitates qPCR repeatability.High compatibility: cDNA product is suitable for application with SYBR Green or Probe qPCR.

Customer Reviews

Product Use Citation(37)

Cell Death Dis, 2018, 9(12):1162
PubMed: 30478301
Front Oncol, 2018, 10.3389/fonc.2018.00629
https://www.frontiersin.org/articles/10.3389/fonc.2018.00629/full
J Neurosci Res, 2018, 96(10):1717-1726
PubMed: 30043506
Pharmaceutical Biology, 2018, 10.1080/13880209.2018.1543331
https://www.tandfonline.com/doi/abs/10.1080/13880209.2018.1543331
Biol Pharm Bull, 2018, 41(11):1638-1644
PubMed: 30135326
Microbes Infect, 2018, 20(2):81-88
PubMed: 29109018
J Gastroenterol Hepatol, 2018, 33(5):1138-1145
PubMed: 29141109
Pharmacol Res, 2018, 129:443-452
PubMed: 29155016
Biochem Biophys Res Commun, 2018, 503(4):2800-2806
PubMed: 30100063
Front Genet, 2018, 9:356
PubMed: 30233644
Exp Cell Res, 2018, 1016/j.yexcr.2018.08.012
PubMed: 30098333
Phytother Res, 2018, 32(8):1521-1529
PubMed: 29672953
Acta Biochim Pol, 2018, 10.1111/pce.13448
PubMed: 29549670
Int J Mol Sci, 2018, 19(4)
PubMed: 29652862
Stem Cells Int, 2018, 2018:7139485
PubMed: 29861742
J Lipid Res, 2018, 10.1194/jlr.M088419
PubMed: 30158201
J Cell Physiol, 2018, 233(5):3945-3954
PubMed: 28884830
Front Physiol, 2018, 9:297
PubMed: 29643817
Cancer Res, 2018, 78(6):1418-1430
PubMed: 29339538
Acta Pharmacol Sin, 2018, 39(1):107-116
PubMed: 28880016
Biomed Pharmacother, 2018, 105:606-615
PubMed: 29890469
J Neuroinflammation, 2018, 15(1):73
PubMed: 29577897
J Surg Res, 2018, 227:198-210
PubMed: 29804854
Exp Cell Res, 2018, 367(1):89-96
PubMed: 29519253
Cell Host Microbe, 2017, 22(1):86-98.e6
PubMed: 28704656
Virology, 2017, 161-169
PubMed: 28441586
FEBS Open Bio, 2017, 7(6):798-810
PubMed: 28593135
Gen Comp Endocrinol, 2017, 10.1016/j.ygcen.
PubMed: 28232027
Am J Physiol Gastrointest Liver Physiol, 2017, ajpgi.00205
PubMed: 28082284
Am J Transl Res, 2016, 8(12):5756-5765
PubMed: 28078047
Int J Oncol, 2016, 48(3):1290-6
PubMed: 26780987
Cell Physiol Biochem, 2016, 40(5):1005-1012
PubMed: 27941341
Cell Res, 2016, 26(12):1302-1319
PubMed: 27801882
J Immunol, 2016, 197(12):4736-4749
PubMed: 27815423
Cell Signal, 2016, 28(11):1735-41
PubMed: 27521603
J Biol Chem., 2016. pii: jbc.M116.718080
PubMed: 27129230
Biochem J, 2015, BJ20150419
PubMed: 26621875

Customer Product Validation(1)

G, EGFR and c-MET mRNA levels in CaSki cell with or without KDM5C restoration. H and I, Ectopic KDM5C expression-mediated eRNA transcription reduction of EGFR and c-MET genes was confirmed by qPCR of three selected super-enhancer–derived eRNAs in the CaSki-vector and CaSki-KDM5C cells. J, mRNA expression of EGFR and c-MET in CaSki- and KDM5C-depleted CaSki cells was examined by RT-qPCR. K and L, The eRNA transcription levels at EGFR and c-MET super-enhancers were examined by qPCR of three selected super-enhancers in parental CaSki- and KDM5C-depleted CaSki cells. In all panels, qPCR data are represented as mean SD from three biological replicates. , P < 0.05; , P < 0.01; t test.

Product Comparison

Click to enlarge
Click to enlarge
Click to enlarge

Comparison of cDNA synthesis using Bimake BioScript All-in-One cDNA Synthesis SuperMix and Invitrogen SuperScript® First-Strand Synthesis System for RT-PCR

Total RNA of Hela cells (10-fold gradient) was used for cDNA synthesis. For Real-Time PCR, 1μl cDNA product was used as template using Bimake™ 2x SYBR Green qPCR Master Mix for detection of high-(ACTB), medium-(B2M), and low-expression (TBP) gene, respectively.

These data show that: Bimake All-in-One presents reliable linear relation within broad range of RNA template concentration and permits high amplification specificity. For amplification efficiency and detection sensitivity, especially at low RNA template concentration, Bimake All-in-One has similar reverse transcription performance compared with Invitrogen SuperScript®.

Price Comparison

Description

BioScript All-in-One cDNA Synthesis SuperMix provides first-strand cDNA synthesis, optimized with the high-temperature capability of Reverse Transcriptase and an optimized SuperMix formula. In one tube, the pre-blended 5× qRT SuperMix contains all the necessary components for reverse transcription of YOUR RNA template. The resulting cDNA product is suitable for use in real-time quantitative RT-PCR (qRT-PCR). Besides, The reverse transcriptase in this kit is purified from E. coli expressing the pol gene of M-MLV, mutagenized to increase thermal stability and cDNA synthesis and to reduce RNase H activity.

Kit Components

Component	B24403	B24408
5×qRT SuperMix *	400 μL	1 mL × 2
5 × No RT ControlMix ♥	40 μL	200 μL
RNase-free Water	1 mL × 2	1mL × 10
* contains Buffer, dNTP, Reverse Transcriptase, RNase inhibitor, Oligo dT/Random primer mix. ♥ contains all the components in 5 × qRT SuperMix, except Reverse Transcriptase.

Application

Synthesis of cDNA from target genes for qRT-PCR, especially low-copy genes or RNA templates of low concentration.

Storage

Store at -20°C for up to 2 years. Repetitive freeze-thaw cycles should be avoided. It is recommended to pre-aliquot the mix into small batches for frequent usage. Product quality is guaranteed under proper storage conditions.

Stability Test

These data showed that, compared to that stored at -20 ℃, after stored at 4 ℃ for 7 days BioScript All-in-One cDNA Synthesis SuperMix showed no significant difference regarding either amplification efficiency or detection sensitivity when used for reverse transcription followed by working with Bimake 2 x SYBR Green qPCR Master Mix.