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Bimake

主营：分子类，蛋白类，抗体类，生化类试剂

当前位置：首页 > 产品中心 > > Bimake/Protein A/G Magnetic Beads for IP/5 mL/B23202

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B23202

产品编号： B23202
美元价： $306.00
市场价： ¥6120.00
品牌： Bimake
产地：美国(厂家直采)
公司： Bimake
产品分类：蛋白类>重组蛋白>其它动物抗原>
公司分类：

联系 Q Q: 1570468124
电话号码: 4000-520-616
邮箱: info@ebiomall.com

Bimake/Protein A/G Magnetic Beads for IP/5 mL/B23202

商品介绍

Advantages
Customer Reviews
Price Comparison
Description
Storage
Protocol
File Download

Advantages

• Spinning free, IP (takes<30 minutes) with minimal sample loss. • Background caused by non-specific binding is very low. • Beads contain 9.3×10¹³ molecules/cm² of protein A/G. Antibody binding capacity up to 0.6-0.8 mg/mL. • Cheap to 73 USD/mL

Customer Reviews

Product Use Citation(18)

Biochem Biophys Res Commun, 2019, 10.1016/j.bbrc.2019.04.016
PubMed: 30979497
J Biol Chem, 2019, 294(15):5945-5955
PubMed: 30782845
Clin Cancer Res, 2019, 10.1158/1078-0432.CCR-18-3791
PubMed: 30862693
Cell Commun Signal, 2018, 16(1):69
PubMed: 30342530
Cancer Res, 2018, 78(18):5274-5286
PubMed: 29945959
Exp Cell Res, 2018, 10.1016/j.yexcr.2018.08.012
PubMed: 30098333
Pharmacol Res, 2018, 128:327-337
PubMed: 29106960
Cell Cycle, 2018, 17(9):1115-1123
PubMed: 29895215
Cancer Res, 2018, 78(12):3190-3206
PubMed: 29700004
Front Microbiol, 2018, 10.3389/fmicb.2018.00094
PubMed: 29467730
Colloids Surf B Biointerfaces, 2018, 161:645-653
PubMed: 29169119
Biochem Biophys Res Commun, 2018, 496(2):621-627
PubMed: 29366480
Neurosci Lett, 2018, 675:133-139
PubMed: 29030221
Virology, 2018, 521:33-43
PubMed: 29879540
Sci Rep, 2017, 7:46238
PubMed: 28387323
Cell Death Dis, 2017, 8(5):e2799
PubMed: 28518143
Invest Ophthalmol Vis Sci, 2017, 58(2):1266-1273
PubMed: 28241314
Cancer Letters, 2016, 376(2): 268-277
PubMed: 27063099

Customer Product Validation(11)

Septin4 is a novel PARP1 interacting protein and the interaction is enhanced under oxidative stress. (A) Immunoprecipitation (IP) and western blotting (IP-western) using anti-PARP1 antibody or negative control IgG and Protein A/G immunoprecipitation magnetic beads followed by anti-Septin4 western blot were performed to ascertain endogenous interaction between PARP1 and Septin4. (B) Anti-Septin4 antibody or negative control IgG and Protein A/G immunoprecipitation magnetic beads followed by anti-PARP1 western blot were performed to ascertain endogenous interaction between Septin4 and PARP1. (C) Endogenous interaction between PARP1 and Septin4 was enhanced by treatment of H2O2, which were evaluated by Immunoprecipitation using anti-PARP1 antibody. (D) Endogenous interaction between Septin4 and PARP1 was enhanced by treatment of H2O2, which were evaluated by Immunoprecipitation using anti-Septin4 antibody.
Exogenous HA-Flag-PHF5A in HEK 293T cells was immunoprecipitated with anti-Flag or control IgG. The immunoprecipitates were then analyzed by Western blot.
The ubiquitination level of Mfn1, Mfn2 and Cx43 in cardiomyocytes which were transfected with gp78-siRNA or Con-siRNA and treated by 5 μM NPS R568 or 3 μM Calhex231 respectively by immunoprecipitation (n= 3). A representative blot is shown. *P<0.05 versus control; # P<0.05 versus HG
(F) Whole-cell lysates from osteosarcoma cell lines were immunoprecipitated with an anti-CSE1L antibody followed by immunoblotting (IB) with anti-MSH6 and anti-CSE1L antibodies. IgG was used as a negative control. (G) Whole-cell lysates from osteosarcoma cell lines were immunoprecipitated with an anti-MSH6 antibody followed by IB with anti-CSE1L and anti-MSH6 antibodies. IgG was used as a negative control.
Immunoprecipitation for c-Myc protein:100 μg of Protein A/G Magnetic beads & 10 μg anti-c-Myc antibody was used.
Immunoprecipitation for Flag Tag-fusion Protein: 1. 1% input 2. Bimake Magnetic Protein A/G beads 3. Bimake Magnetic Protein A/G beads + Mouse Anti-Flag Antibody 4. Life technology Magnetic Protein A/G beads + Mouse Anti-Flag Antibody + IP 5. Bimake Magnetic Protein A/G beads + Mouse Anti-Flag Antibody + IP 6. Santa cruz Protein A/G Agarose gel + Mouse Anti-Flag Antibody + IP
Immunoprecipitation for HAS: 1. 5 ug of rabbit polyclonal antibody was used 2. 5 ug of mouse IgG1 monoclonal antibody was used
Immunoprecipitation for GST-Tag Fusion Protein: 100 ug of Bimake Protein A/G Magnetic beads & 10 ug Anti- GST-Tag Antibody was used
(I and J) 293T cells were co-transfected with a HA-tagged TGEV S1 expression plasmid together with GFP-tagged EGFR Receptor 1 or GFP-tagged EGFR Receptor 2 expression plasmid, cell lysates were immunoprecipitated with an anti-HA antibody or an anti-GFP antibody, the resulting precipitates were examined by immunoblotting using an anti-HA or an anti-GFP antibody to examine the interaction between HA-TGEV S1 and GFP-tagged EGFR. (** p < 0.01).
Immunoprecipitation for HAS: 100 ug of Bimake Protein A/G Magnetic beads & 10 ug Anti-HAS Antibody was used
Immunoprecipitation for GST-Tag Fusion Protein: 1. Antigen & Anti-GST-Tag Antibody (Mouse IgG1) 2 & 3. Elution after IP 4. Antigen & Anti-GST-Tag Antibody (Mouse IgG1) 5 & 6. Elution after IP (Control group without GST-Tag Fusion Protein)

Price Comparison

Description

Protein A/G Magnetic Beads for IP use a biological nanosurface technology (S-TEC). Protein A/G is orientated as a coat on the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10¹³ molecules/cm². Compared to other similar immune magnetic beads, Bimake MagBeads™ Protein A/G display more antibody binding sites, therefore during IP, less magnetic beads are used. Non-specific binding is low, enabling Bimake MagBeads Protein A/G to be used in IP conveniently and efficiently. With a large, specific surface area, these beads can greatly shorten the equilibrium antibody and antigen adsorption time, enabling complete antibody antigen adsorption process within 10 minutes, and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens.

Specificity

Protein A/G is a recombinant fusion protein that combines IgG binding domains of both Protein A and Protein G. Protein A/G contains four Fc binding domains from Protein A and two from Protein G, yielding a final mass of 50,460 daltons. The binding of Protein A/G is less pH-dependent than Protein A, but otherwise has the additive properties of Protein A and G. Table1 provides a reference of binding strength between antibodies and protein A/G, use the antibody subtype with strong binding effect in your experiment.

Properties

Concentration	10 mg/mL
Particle size	100 nm
Binding capacity of human IgG	0.4-0.5 mg/mL
Ligand	Recombinant protein A/G
Application	rProtein Purification, Immunoprecipitation
pH stability	6-8 (Long term)
Shipped in	Wet ice

Storage

Store at 4°C for 2 years. Don’t freeze in the absence of glycerol.

Protocol

This procedure (Figure 1) offers a general guideline for immunoprecipitation (IP). Optimization may be required for each antibody and target antigen. Protein A/G Magnetic Beads for IP are ideally suited for IP reactions.

Figure 1 General Protocol for Immunoprecipitation

Recommended buffer examples

Table 2. Recommended buffer examples
Buffer	Contents (Prepared by customers)
Binding buffer	50 mM Tris, 150 mM NaCl, 0.1%-0.5% detergent (TritonX-100, Tween 20 or NP40), pH 7.5
Wash buffer	50 mM Tris, 150 mM NaCl, 0.1%-0.5% detergent, pH 7.5
Elution buffer	0.1 M -0.2 M Glycine, 0.1%-0.5% detergent, pH 2.5-3.1 (or 0.1 M citric acid, 0.1%-0.5% detergent, pH 2.5-3.1)
Neutralize buffer	1M Tris, pH8.0

Important Notes before Beginning

1 Before immunoprecipitation, please be sure to carefully read the operating instructions. 2 This product requires use of a magnetic separator. 3 Protein A/G Magnetic Beads should be suspended uniformly before use. 4 Protein A/G Magnetic Beads should be kept in storage solution and prevent dry. 5 Do not freeze or centrifuge MagBeads protein A/G. 6 In order to ensure the best results, please select an antibody with strong specificity. 7 For the IP experiments, different antibodies and antigens will display different binding affinities. Antibody and antigen binding may be altered based on use of binding buffer and washing buffer. Some operator optimization may be necessary. 8 This product is only intended to be used as directed. All other uses are prohibited.

Table 1. Relative Binding Strengths of Antibodies to Protein A and Protein G
Species	Antibody class	sProtein A/G	sProtein A
Human	Total IgG	＋＋＋＋＋	＋＋＋＋＋
	IgG1, IgG2	＋＋＋＋＋	＋＋＋＋＋
	IgG3	＋＋＋＋＋	＋
	IgG4	＋＋＋＋＋	＋＋＋＋＋
	IgM	＋	＋
	IgD	－	－
	IgA	＋	＋
	IgA1, IgA2	＋	＋
	IgE	＋＋＋	＋＋＋
	Fab	＋	＋
	ScFv	＋	＋
Mouse	Total IgG	＋＋＋＋＋	＋＋＋＋＋
	IgM	－	－
	IgG1	＋＋＋	＋
	IgG2a	＋＋＋	＋＋＋
	IgG2b	＋＋＋	＋＋＋
	IgG3	＋＋＋	＋＋＋＋＋
Rat	Total IgG	＋＋＋	＋
	IgG 1	＋＋＋	＋＋＋
	IgG2a	＋＋＋＋＋	＋＋＋
	IgG2b	＋	＋＋＋
	IgG2c	＋＋＋＋＋	＋＋＋
Cow	Total IgG	＋＋＋＋＋	＋
	IgG1	＋＋＋＋＋	＋
	IgG2	＋＋＋＋＋	＋＋＋＋＋
Goat	Total IgG	＋＋＋＋＋	＋
	IgG1	＋＋＋＋＋	＋
	IgG2	＋＋＋＋＋	＋＋＋＋＋
Sheep	Total IgG	＋＋＋＋＋	＋
	IgG1	＋＋＋＋＋	＋
	IgG2	＋＋＋＋＋	＋＋＋＋＋
Horse	Total IgG	＋＋＋＋＋	＋
	IgG(ab), IgG(c)	＋	－
	IgG(T)	＋＋＋＋＋	＋
Rabbit	Total IgG	＋＋＋＋＋	＋＋＋＋＋
Guinea pig	Total IgG	＋＋＋＋＋	＋＋＋＋＋
Hamster	Total IgG	＋＋＋	＋＋＋
Pig	Total IgG	＋＋＋＋＋	＋＋＋＋＋
Donkey	Total IgG	＋＋＋＋＋	＋＋＋
Cat	Total IgG	＋＋＋＋＋	＋＋＋＋＋
Dog	Total IgG	＋＋＋＋＋	＋＋＋＋＋
Monkey	Total IgG	＋＋＋＋＋	＋＋＋＋＋
Chicken	Total IgG	－	－