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Bimake

主营：分子类，蛋白类，抗体类，生化类试剂

当前位置：首页 > 产品中心 > > Bimake/Phosphatase Inhibitor Cocktail (2 Tubes, 100X)/1 mL A + 1mL B/B15001

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B15001

产品编号： B15001
美元价： $54.00
市场价： ¥1080.00
品牌： Bimake
产地：美国(厂家直采)
公司： Bimake
产品分类：细胞相关>细胞培养>刺激/抑制试剂>
公司分类：

联系 Q Q: 1570468124
电话号码: 4000-520-616
邮箱: info@ebiomall.com

Bimake/Phosphatase Inhibitor Cocktail (2 Tubes, 100X)/1 mL A + 1mL B/B15001

商品介绍

Customer Reviews
Price Comparison
Description
Storage
Protocol
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Customer Reviews

Product Use Citation(54)

Cancer Sci, 2019, 110(4):1268-1278
PubMed: 30689267
Biochim Biophys Acta Mol Cell Res, 2019, 1866(8):1249-1259
PubMed: 30951783
Mol Metab, 2019, 22:21-36
PubMed: 30795914
Acta Pharmacol Sin, 2019, 10.1038/s41401-019-0214-z
PubMed: 30792486
Autophagy, 2019, 10.1080/15548627
PubMed: 30957628
FASEB J, 2019, 33(4):4780-4789
PubMed: 30601680
J Transl Med, 2019, 17(1):107
PubMed: 30940158
Am J Physiol Endocrinol Metab, 2019, 316(3):E510-E518
PubMed: 30620634
DNA Cell Biol, 2019, 10.1089/dna.2018.4521
PubMed: 30835546
Acta Neuropathol, 2019, 10.1007/s00401-019-01967-4
PubMed: 30729296
Cell Rep, 2019, 26(5):1273-1285
PubMed: 30699354
Cell Mol Immunol, 2018, 10.1038/s41423-018-0183-z
PubMed: 30523347
Mol Neurobiol, 2018, 10.1007/s12035-018-1445-6
PubMed: 30565051
Mol Pharmaceutics, 2019, 10.1021/acs.molpharmaceut.8b00870
https://pubs.acs.org/doi/abs/10.1021/acs.molpharmaceut.8b00870
Int Immunopharmacol, 2019, 66:146-153
PubMed: 30453148
J Clin Invest, 2018, 128(9):4148-4162
PubMed: 29920190
Cancer Res, 2018, 78(18):5274-5286
PubMed: 29945959
Cancer Res, 2018, 10.1158/0008-5472.CAN-17-1394
PubMed: 30093560
Mol Psychiatry, 2018, 10.1038/s41380-018-0086-5
PubMed: 29910467
Am J Physiol Renal Physiol, 2018, 10.1152/ajprenal
PubMed: 29897282
Cell Signal, 2018, 52:74-82
PubMed: 30176291
J Hematol Oncol, 2018, 11(1):77
PubMed: 29866190
J Mol Histol, 2018, 49(5):459-469
PubMed: 30014245
Mol Immunol, 2018, 99:191-198
PubMed: 29807327
J Cell Mol Med, 2018, 10.1111/jcmm.13665
PubMed: 29761889
Biochem Biophys Res Commun, 2018, 497(2):473-479
PubMed: 29408378
Biochem Biophys Res Commun, 2018, 501(4):1068-1073
PubMed: 29777710
Int J Mol Sci, 2018, 19(4)
PubMed: 29652862
Oncol Lett, 2018, 16(4):4439-4447
PubMed: 30214578
Transl Oncol, 2018, 11(5):1160-1170
PubMed: 30056367
J Neurosci Res, 2018, 10.1002/jnr.24324
PubMed: 30216497
J Cell Mol Med, 2018, 22(9):4423-4436
PubMed: 29993186
Sci Rep, 2018, 8(1):773
PubMed: 29335505
J Exp Clin Cancer Res, 2018, 37(1):189
PubMed: 30103781
J Ethnopharmacol, 2018, 214:141-152
PubMed: 29247698
J Cell Physiol, 2018, 233(4):3066-3079
PubMed: 28681913
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PubMed: 29411534
Int Urol Nephrol, 2018, 50(4):675-686
PubMed: 29460131
Int J Mol Med, 2018, 42(2):851-860
PubMed: 29750298
Exp Gerontol, 2018, 110:284-290
PubMed: 29958998
J Hematol Oncol, 2018, 11(1):77
PubMed: 29866190
Mol Immunol, 2018, 99:191-198
PubMed: 29807327
Am J Physiol Endocrinol Metab, 2018, 10.1152/ajpendo.00050.2018
PubMed: 29763374
Virology, 2017, 507:161-169
PubMed: 28441586
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PubMed: 28588028
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PubMed: 28007785
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PubMed: 27404702
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PubMed: 27316715
Sci Rep, 2016, 6:32886
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Customer Product Validation(14)

Liver tissue samples from WT (W1, W2, and W3) and Hectd3–/– mice (H1, H2, and H3) on day 2 after infection with F. novicida and uninfected WT (W) and Hectd3–/– (H) mice were homogenized, and lysates were analyzed for activation and expression of caspase-3, caspase-1, caspase-11, ZBP1, and HECTD3, and phosphorylation of IκBα, STAT1, and STAT3. GAPDH was used as loading control. Each symbol indicates an individual mouse.
β-Catenin and pGSK-3β proteins levels normalized to β‐Actin (40 KD) after 8 hrs of incubation in NTM cells following exposure to different concentrations of ODM and RPE EVs. Total protein was extracted from NTM cells and analysed by Western blot.
Immunoblot analysis of liver samples from WT-Con (n=2), WT-ETOH (n=6), and Tg-ETOH (n=6) mice exposed to the chronic (6 weeks) ethanol containing diet protocol. phosphatase inhibitors are bought from Bimake (Cat# B15001).
The expression levels of EMT-associated markers in miR-616-3p overexpression or knockdown cell lines were detected by Western blot analysis. Protease inhibitor is purchased from Bimake.
Western blot was used to detect the protein level of Regnase-1 in NIH3T3 cells treated with BRD4 inhibitor JQ1 and I-BET-762. Protease inhibitor (Bimake, Cat# B14001).
Western blotting evaluated the expression levels of AKT, p-AKTT308, and p-AKTS473 in si-LINC00470-transfected GBM cells. Protease inhibitor cocktail (Bimake, B14001) and phosphatase inhibitor cocktail (Bimake, B15001).
Knockdown of SMYD2 by siRNAs impaired SMAD1/5 phosphorylation induced by BMP2. HaCaT cells were transfected with SMYD2 siRNAs (siSMYD2-1 and 2). Two days later, the cells were serum starved overnight and stimulated with BMP2 for 0, 15, 30 and 45 minutes as indicated, and the levels of SMAD5, phosphorylated SMAD1/5 (SMAD1/5ph) and SMYD2 were analyzed by Western blotting. GAPDH was shown as the loading control.
Western blotting was used to detect pY701-STAT1, STAT1, pY690-STAT2, STAT2, and FLAG-VP3 expression in HEK293 cells
Bimake cocktails protect phosphorylated AKT and ERK better than their competitor.
The 100X solution performs as well as Roche tablets in protecting total and phosphorylated p38.
Bimake cocktails allow for detectable phosphorylated AKT after 7 hours of immuno-precipitation.
Bimake inhibitor cocktails perform the best as compared to their competitors!
Bimake allows for detectable phosphorylated-AKT for 7 hour incubation at room temperature in 293 cells.
Bimake allows for detectable phosphorylated-AKT for 16 hour incubation at 4 degrees in 293 cells.

Price Comparison

Price Contrast Calculation

Brand	Cat. No.	Type	Packaging	Price	Work Volume (1x)	1 rxn PRICE (for 1mL lysis system)	Small Package (100mL 1x)	Large Package (1000mL 1x)
Bimake	B15001	2 Tubes (A in H₂O, B in DMSO)	1mL A + 1mL B (100x)	37 USD	100mL	0.37 USD	37 USD
Sigma	P5726-1ML	Aqueous solution	1mL(100x)	65 USD	100mL	0.65 USD	65 USD
PIERCE	78420	Aqueous solution	1mL(100x)	107 USD	100mL	1.1 USD	107 USD
Calbiochem	524625-1ML	Aqueous solution	1mL(100x)	120 USD	100mL	1.2 USD	120 USD
Roche	04906845001	Tablets	10 Tablets (10x per tablet)	150 USD	100mL	1.5 USD	150 USD
Bimake	B15002	2 Tubes (A inH₂O, B in DMSO)	1mL A + 1mL B (100x)x 10	270 USD	1000mL	0.27 USD		270 USD
Sigma	P5726-5ML	Aqueous solution	5mL(100x)	222 USD	500mL	0.44 USD		444 USD
PIERCE	78427	Aqueous solution	10mL(100x)	730 USD	1000mL	0.73 USD		730 USD
Calbiochem	524625-1SET	Aqueous solution	1mL x 5(100x)	468 USD	500mL	0.94 USD		936 USD
Roche	04906837001	Tablets	20 Tablets (10x per tablet)	180 USD	200mL	0.90 USD		900 USD

Description

For Phosphorylation Protection, more inhibitors mean better results. So we provide Two Tubes with 8 inhibitors. Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. For decades, researchers have used easily-accessible phosphate mimetics (tube A) to prevent the loss of phosphorylation on biomolecules in vitro. In recent years however, a number of naturally derived agents (tube B) have been discovered as potent inhibitors of phosphatases, thus increasing the available protection spectrum.

Tube #	Ingredient	100x Conc.	Target
A (Aqueous)	Sodium Fluoride	100 mM	Acid phosphatases
	Sodium Orthovanadate	100 mM	Alkaline phosphatases, PTPs, ATPases
	Sodium Tartrate	400 mM	Acid phosphatases
	Sodium Molybdate	115 mM	Acid and phosphoprotein phosphatases
	Imidazole	200 mM	Alkaline phosphatases
B (DMSO)	(-)-p-Bromotetramisole oxalate	2.5 mM	Alkaline phosphatases
	Cantharidin	500 μM	Ser/Thr phosphatases
	Microcystin LR, Microcystis aeruginosa	500 nM	PP1 and PP2A

Works well in common detergents such as 1% SDS, Triton, and NP-40 DTT, EDTA and EGTA may reduce the protection effects of Sodium Orthovanadate

Storage

The product should be stored at -20°C, and is stable for up to 2 years. When kept at 2-8°C, the product remains stable for 2 months.

Protocol

Application Fields	Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Experiment Protocol	Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Note: For optimum protection of phosphorylated proteins and lipids, we recommend simultaneous use of BOTH Tubes A and B. Because the inhibitor cocktails target different families of phosphatases, synergistic stabilization effects are observed with dual-treatment.

The Phosphatase Inhibitor Cocktail does not contain protease inhibitors. Therefore,we recommend that a protease inhibitor cocktail is added beforehand. Protease inhibitor cocktails are frequently used in mammalian cell lysates or tissue extracts to increase protein stability. As the name indicates, the cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates.

Trouble Shooting

Q#1: Weak western bands for both phosphorylated and non-phosphorylated proteins.A#1: Add a protease inhibitor cocktail beforehand to protect all kinds of proteins. Bimake provides high quality protease inhibitor cocktails at competitive prices.Q#2: Light-green sediment precipitated after mixing two tubes before use.A#2: This sediment is mainly salt precipitation. Salts in the aqueous tube and natural compounds in the DMSO tube will both be influenced by mixing. The precipitate cannot be completely re-dissolved and mixing the two tubes should be avoided. Separate addition of the two tubes is required as described in the instructions.Q#3: Tube B takes a long time to melt.A#3: This is because DMSO thaws very slowly, especially in low temperatures. Thawing in 25℃ water bath is recommended. Dividing Tube B solution into 100ul aliquots can shorten the thawing time.